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Chemical and enzymatic approaches to peptide ligation are important alternatives of recombinant DNA technology for protein synthesis and modification.
The rfOmpC also sets a stage for rational design by recombinant DNA technology for vaccine design and high resolution structure determination.
The company uses a "stool-based" DNA technology for early detection of colorectal cancer.
For future clinical trials, we have constructed human fVII proteins with a lys341Ala mutation, similarly by recombinant DNA technology, for fVII-tPDT for patients with cancer or wMD.
A novel trend is to apply recombinant DNA technology for producing lectins with improved characteristics for construction of various lectin-based biodevices.
To investigate LOH of BRCA1 (17q21) and BRCA2 (13-q12-13) in sporadic breast cancer, polymerase chain reaction (PCR -based fluorescent DNA technology for detection of microsatellite PCR -basedsms was applied.
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New human papillomavirus (HPV) DNA technologies for the detection and prevention of cervical cancer have led to exciting changes in cervical cancer screening worldwide.
cDNA was created by first incubating 5 μg of fragmented RNA in a 28 μl solution containing dNTP (Thermo Fisher Scientific) containing a custom biotinylated oligo dT primer (integrated DNA Technologies) for 5 min at 65 °C.
While these studies provide empirical evidence of the relevance and value of DNA technologies for criminal investigation, they also expose the problematic aspects of engaging in an empirical study of this matter, like sample selection bias or generalizability of the results.
Standard recombinant DNA technologies for gene cloning [ 31] were applied.
With advances in mRNA profiling and detection techniques, we have established a novel and non-radioactive bioassay system using branched DNA (bDNA) technology for detecting protein-therapeutic neutralizing antibodies in patient serum.
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