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The MB and synthetic DNA targets were purchased from FASMAC Co. Ltd. (Atsugi, Kanagawa, Japan).
The DNA hybridization to complementary DNA targets labelled with fluorescent markers was confirmed using fluorescence microscopy.
MABS uses a unique property of bifunctional type II REs to methylate DNA targets they recognize.
As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected.
Microarray technology offers parallel analysis of a high number of DNA targets.
The harder charge modulation makes it more difficult for the DNA targets to induce an electrical signal.
Engineering zinc finger protein motifs for specific DNA targets in genomes is critical in the field of genome engineering.
Nuclear entry of oligonucleotides and enhancement of their affinity to the DNA targets are key points of such approaches.
The technology utilizes gold nanoparticles derivatized with thiol modified oligonucleotides that are designed to bind complementary DNA targets.
A strategy allowing for amplification, detection and genotyping of different genomic DNA targets in a single reaction container is described.
Upon the recognition of a point mutation DNA targets, the padlock probe was ligated to generate a circular template.
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