Sentence examples for dna target with from inspiring English sources

Interestingly, on the basis of our results we can argue that also the truncating substitution C261X, changing the first cysteine of ZF4 into a stop codon, generates a protein unable to recognize the DNA target with adequate affinity because it is missing the entire C-terminus, including the DNA-contacting ZF4 domain.

The hybridization of the complementary fluorescein-labeled DNA target with the surface-bound oligonucleotides was completed within 20 s upon heating with low-power microwaves.

The α3 helix forms the recognition helix, which slots into the major groove of the DNA target with a GGA core (Figure 2a).

The use of a fluorogenic substrate enabled the detection of a single-stranded DNA target with a 1 nmol L−1 detection limit.

Melting points of synthetic DNA target with varied percentages (from 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 to 100%) of the mutation type of single insertion relative to the perfect match templates using Indel_3probe.

The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA.

Measurements of binding constants show that HES-1 recognizes dsDNA synthetic oligonucleotides corresponding to several functional DNA targets with high affinity, but with relatively little specificity.

We further show that the alterations of the FBKs of Cy5 on probe hybridization can be used to differentiate DNA targets with single-nucleotide differences.

Here, a biochip was used to detect synthetic viral DNA targets with detection limit of 100 pM.

Moreover, some transcription factors and co-factors bind to methylated and non-methylated DNA targets with distinct sequences.

All agreed that this is an important model system for both a general understanding of how multiprotein complexes assemble on DNA targets with well-separated binding sites and where supercoiling is involved, as well as more specifically for serine recombinase-family proteins.