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Hybridization assays with the DNA target were performed in a complex medium by cyclic voltammetry.
Structural origins of DNA target selection and nucleobase extrusion by a DNA cytosine methyltransferase.
A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites.
Sternberg, S. H. et al. Conformational control of DNA target cleavage by CRISPR Cas9.
How such complexes recognize and gain access to their DNA target sites is not known.
DNA target regions involved in I-CreI_D75N and I-CreI_3115 binding or catalysis.
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Dardaei, L. et al. Meis1 overexpression causes a change of DNA target-sequence specificity which allows binding to the AP-1 element.
Since different subsets of protein-DNA target contacts may be sufficient to maintain a high degree of sequence-specific homing site recognition and cleavage, some I-CreI-DNA target interactions may be altered and additional changes may be accommodated5.
This technique is based on biochemical interaction of antigen-antibody or probe DNA-target DNA hybridization.
Effective endonuclease activity of TALEN requires dimerization of two monomers that recognize individual DNA target sites.
Labeled DNA target was prepared according to the Affymetrix Mapping 500 K protocol.
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