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Cloning into a single-stranded M13 phage results in single-stranded DNA suitable for sequencing.
Cloning into single-stranded M13 phage results in single-stranded DNA suitable for sequencing.
This protocol produces an optimal yield of high-purity DNA, suitable for genotyping.
We compared various methods for sample processing and recovery of extrachromosomal DNA suitable for qPCR.
Paterson, A. H., Brubaker, C. L. & Wendel, J. F. A rapid method for extraction of cotton (Gossypium spp). genomic DNA suitable for RFLP or PCR analysis.
Findings presented here demonstrated that the more automated methods were successful in extracting DNA from both high- and lower-biomass biofilm samples scraped from the inner surface of oil pipelines and that all three extraction platforms produced high-quality DNA suitable for PCR-based analyses.
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This test improves on previous nested PCR protocols and can be used for the discrimination of samples with <20 pg of template DNA, thus suitable for DNA extracted from horn products.
The proposed acceptance criteria for assessment of DNA quality are hence intended to 'filter' for those DNA extracts suitable for downstream qPCR application.
When a DSB is processed for repair by HRR, the 5' DNA ends are resected to provide 100-200 nucleorides or more of 3' DNA overhangs suitable for strand-invasion, Holliday junction formation, and the completion of repair [ 42].
It is demonstrated that long-range coherent charge transport along the DNA structure is supported by mobile polaron and breather solutions, suggesting that DNA seems suitable for the design of functional nanostructures as ingredients in molecular nanoelectronic devices.
Whole genome DNA libraries suitable for sequencing on Illumina's sequencing platform were generated from 5 μg of genomic DNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com