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Similarly, mitochondrial DNA studies based on RFLPs have indicated very low levels of sequence divergence between these species [ 54], and sequencing of 614 bp of non-coding mtDNA in this study also revealed the three tropical apomicts to be very similar.
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Fragmentation of FFPE DNA is a real drawback, particularly in DNA methylation studies based on methylation-independent PCR: the design of the primers must be conducted with the highest score, primers have to hybridize out of the CpG islands, and amplicon length has to be limited.
DNA replication studies based on incorporation of BrdU or tritiated thymidine present blurred pictures of the temporal-spatial patterns of mitotic activity because an extended period of time intervenes between application and visualization of the probe.
We argue that it should be preferred over DNA barcoding and metagenomic studies based on a single marker for a variety of organisms for which the definition of species is ambiguous (prokaryotes and eukaryotes alike).
These inferences resulted from studies based on DNA-DNA hybridization or poorly assembled and fairly low coverage genomes, however, and currently available genomic resources could provide new insights into the evolutionary history of Rsp repeat family evolution.
The same group recently [ 16] reported an applied DNA pooling study based upon the 10 K Array with mild mental impairment as a phenotype.
Studies based on DNA hybridization suggested years ago that the then-current classification of birds was incorrect in many ways.
The phylogenetic relationships of the aquiline eagles are incompletely known, but two recent studies based on DNA sequence data both place Harpia near the base of this radiation (Helbig et al. 2005, Haring et al. 2007).
Several studies based on DNA SNP marker panels showed that genetic variation between substrains is present [ 4- 6].
In brief, our results were broadly consistent with previously phylogenetic studies based on DNA hybridization [ 44], Cytb [ 45] and vocalization [ 40].
In recent phylogenetic studies based on DNA data alone, a monophyletic Tineoidea has only been obtained in the studies by Regier et al. [ 10] and [ 55] in analyses of unaltered nucleotides from all three nucleotide positions (nt123).
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