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The ability of VRR-Nuc domains to bind a range of DNA structures was examined using electrophoretic mobility-shift assays.
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All synthesized DNA structures were analyzed with agarose gel electrophoresis (1.2 %).
Their binding, folding and stabilizing abilities to human telomeric G4 DNA structures were comparatively researched.
The location and distribution of these DNA structures were examined in different times during the whole uptake process (Fig. 4).
Among these models, flap-shaped DNA or splayed duplex DNA structures are expected to exist during the multiple steps of repair.
G-quadruplex DNA structures are hypothesized to be involved in the regulation of gene expression and telomere homeostasis.
Ligands that stabilize these G-quadruplex DNA structures are potential inhibitors of the cancer cell-associated enzyme telomerase.
All synthesized DNA structures were analyzed with agarose gel electrophoresis (1.2 %), and part of typical products was measured by dynamic light scattering.
As a newly developed drug delivery strategy, nano-sized DNA structures are of low cost, high stability, and feasibility to synthesize; meantime, it is bio-safe due to the lack of exogenous immune activity.
A gallery of zoomed images of convoluted DNA structures is shown in Figure 2D.
Consequently, three new aptamers with the quadruplex/duplex DNA structures are produced as the second generation.
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