Sentence examples for dna structures including from inspiring English sources
The GAATTC sequence is capable of adopting multiple non-B DNA structures including YRY and RRY triplexes.
MEN1 also associates with a variety of DNA structures, including Y-structures, branched structures, and 4-way junction structures [ 68].
PARP-1 binds to other non-B DNA structures including hairpin, cruciform, and loop, and is catalytically activated [ 38].
Unlike most of the other helicases, RecQ family helicases preferentially resolve a broad type of DNA structures, including forked duplex, HJ, G4, D-loop and DNA bubbles.
Firstly, budding yeast Mus81-Mms4 (human MUS81-EME1), an XPF-family heterodimeric endonuclease, was shown to process a broad range of branched DNA structures, including HJs [16,17].
Several structure-selective nucleases (Mus81– Mms4 heterodimer, Yen1 and Slx1– Slx4 heterodimer) have been shown to cleave branched DNA structures, including HJs, in vitro (Boddy et al. 2001; Kaliraman et al. 2001; Fricke and Brill 2003; Ip et al. 2008).
Modifications include adding agents known to relax DNA structure including DMSO, Betaine, PCRx Enhancer and ThermoFidelase I; and adding increasing amounts of dGTP BigDye terminator (dGTP) chemistry to the standard BigDye v1.1 (BD) chemistry which contains dITP rather than dGTP.
Freier and Altmann described some 200 experimental modifications of DNA structure, including modifications of the base, sugar, and backbone, and their impact on hybridization to complementary RNA strands as measured by change in melting temperature (Tm) (Freier and Altmann, 1997).
Sequence-specific DNA-binding enzymes such as methyltransferases (MTases) and endonucleases comprising bacterial restriction modification (R/M) systems would seem to present excellent targets for analysis via fusion to GFP given that many of them introduce complex rearrangements of DNA structure including for example DNA looping to bring distant sites on a single DNA molecule into close proximity.
The discovery of DNA secondary structures, including quadruplexes in G-rich DNA (G4s) and iMs in C-rich DNA (iMs), has enabled the diversification of nucleic acid uses from their original roles in conventional biological processes to building blocks for nanoscale composite materials.
AATTTA repeats have been characterized in vitro to form various types of non-B DNA secondary structures, including hairpin, triplex, non-H DNA [8], [9], [10], [11], which potentiallychallenge the stable maintenance of the repeats in genomes [8].
