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Regardless of source, DNA specimens were quantified using human-specific Alu-PCR to confirm a minimum level of amplifiable human DNA [ 17] and randomized prior to genotyping.
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The concentration of the protein extracted from each specimen was quantified using the BCA protein assay kit.
The extracted DNA specimens were stored at −20°C until used.
DNA specimens were extracted and genotyped on the Ilmn650Y array.
DNA specimens were screened using two nested PCR assays.
DNA specimens were collected under Institutional Review Board-approved protocols.
The possibility of DNA fingerprinting using cell-free fetal DNA specimens was also confirmed.
The DNA samples were quantified by Nano Drop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA).
DNA samples were quantified using picogreen.
DNA concentrations were quantified using NanoDrop spectrophotometer (NanoDrop Technologies Inc ,Wilmington, DE, USA).
All DNA stocks were quantified by organism-specific qPCR.
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