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This technique was more successful when frozen DNA specimens were analyzed than when DNA extracted from paraffin-embedded samples was used, 99.1% versus 93.3% (p<0.001).
Purified DNA specimens were analyzed for CpG methylation of the PMEPA1 gene.
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Insufficient DNA in the extracted sample or degraded DNA may offer an alternative explanation for the negative results since some specimens were analyzed even eight months after collection.
For some specimens, DNA extraction, PCR amplification, and sequencing took place during the cruise; other specimens were analyzed at the University of Connecticut.
Fifteen arterial specimens were analyzed by nine laboratories using 16 PCR techniques, with the detection of C. pneumoniae DNA in at least one specimen by six of nine laboratories [ 14].
(A) Tumor specimens were analyzed by IHC staining for PD-L1, using DAB Peroxidase (HRP) staining technique.
The specimens were analyzed using micro-CT.
The differences between multiple specimens were analyzed when necessary.
Urine and blood specimens were analyzed in these children.
After mounting, the specimens were analyzed under light microscopy.
Specimens were analyzed at the University of Zimbabwe laboratories.
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