METHODS: Because early-stage ovarian cancers are relatively uncommon, we adapted p53 immunostaining and DNA sequencing methods for use in paraffin-embedded tissue blocks.
For his contributions to DNA sequencing methods, Sanger shared the 1980 Nobel Prize for Chemistry.
DNA sequencing methods, including whole genome sequencing and multilocus sequence analysis (MLSA), likewise have proved useful in the identification of bacteria.
Using advanced DNA sequencing methods, researchers have identified a new gene that is associated with sporadic amyotrophic lateral sclerosis (ALS), or Lou Gehrig's disease.
Current DNA sequencing methods rely on sequencing millions of events at the same time to generate a large enough signal and determine the underlying sequence.
Most DNA sequencing methods are also generally incapable of identifying chemically modified bases arising from DNA damage.
The first DNA sequencing methods were invented in the late 1970s, but pure DNA, ready for sequencing, was difficult to produce thus, making DNA sequencing labor- and time-intensive compared to other tools for making indirect inferences about genetic sequences.
The ability of the designed molecular beacon to specifically detect the T790M mutation of EGFR was tested for samples from two patients with drug resistance and compared with conventional DNA sequencing methods.
A case control study of 126 patients and 198 controls was performed to investigate the association between Survivin A9194G polymorphisms and PTC susceptibility by polymerase chain reaction (PCR) following DNA sequencing methods.
In this review, we present an overview of the state-of-the-art single-cell DNA sequencing methods, technical errors that are inherent in the resulting large-scale datasets, and computational methods to overcome these errors.
Given that DNA sequencing methods are time-consuming and insensitive, we developed and investigated the feasibility of using molecular beacons for the detection of the T790M mutation in EGFR.
