For the same reason, the region spanning position 316 to 382 in the coding DNA sequence alignment (positions 280-303 in AY497771) consisting of gaps and poorly aligned sequences were removed.
DNA sequence alignment was performed using BWA-MEM.
Course Notes: Weekly computer laboratories will introduce the use of the internet and computational software in DNA sequence alignment and phylogenetic and population genetic analysis.
In order to identify the presence of pathogens in past populations we used a novel high-throughput DNA sequence alignment and taxonomic assignment tool MALT (MEGAN ALignment Tool) and were able to identify traces of Salmonella enterica DNA in individuals buried in an early contact era epidemic cemetery at Teposcolula-Yucundaa, Oaxaca in Southern Mexico.
All plasmids constructed in this study were subjected to DNA sequencing before use, and DNA sequence alignment was carried out with Vector NTI (Invitrogen).
The donor-specific SNPs at the promoter and the third exon were represented through DNA sequence alignment among 15 varieties, including Nipponbare, two different OsSPL14 donors (ST12 and Aikawa1), and 12 recipients.
Fig. 1 DNA sequence alignment of ITS regions. 1 Cyclaneusma niveum type strain CBS 495.73, 2 Cyclaneusma minus type strain CBS 496.73, 3 C. minus NZFS 4257 ('verum'), 4 C. minus NZFS 4114 ('verum'), 5 C. minus NZFS 4263 ('simile'), 6 C. minus NZFS 4129 ('simile'simile
Primer design was based on a DNA sequence alignment of M13K07, VCSM13 and fUSE5 (Fig. S4).
DNA sequence alignment, coding region assembly, and in silico translation were done using the DNAsis Max program 2.0 (Hitachi).
DNA sequence alignment is a prerequisite to the identification of virtually all conserved sequence motifs and estimation of evolutionary divergence between sequences.
However, other technical considerations are likely to be at least as important, such as the ease of sequence recovery, the frequency of DNA sequencing artifacts, and the question of whether DNA sequence alignment is useful for identifying species using barcodes.
