DNA was removed with RapidOut DNA Removal Kit (Thermo Scientific).
If it is white, then it may still have salts or other naturally occurring contaminants and you can repeat the ethanol wash a second time; however, the DNA removal step below seems to also remove salt contamination.
Residual DNA was completely removed using QIAGEN's RNase-Free DNase Set and complete DNA removal was validated by performing PCR with the B. pseudomallei recA gene primers.
This was followed by DNA removal using DNase I (Sigma Aldrich).
All metagenomes analysed here were obtained from the SRA after human DNA removal by the SRA using BMTagger (Extended Data Fig. 7a).
Four hours after LPS or vehicle treatment, RNA isolation was performed using TRIzol (Thermo Fisher Scientific, Waltham, MA, USA) and DNA removal was performed with Ambion DNA removal kit (Thermo Fisher Scientific). 1 µg of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Biorad) according to the manufacturer's instruction.
DNA removal in RNA samples is essential to prevent the incorporation of any genomic material in the RNA-Seq library and consequently the misinterpretation of RNA-Seq data analyses.
List of soil sample locations, ecosystem descriptions, edaphic characteristics, mean community dissimilarity after relic DNA removal, mean percent of relic DNA for each soil, mean richness, and mean 16S and ITS amplicon abundances for both untreated and PMA-treated soils.
A number of difficult challenges have to be overcome to achieve this goal that include protein characterization, carbohydrate mapping, DNA removal, establishment of genetic stability, retrovirus clearance, and identification of host-cell contaminants.
Rat aortas were decellularized using a sequential combination of weak detergents followed by a nuclease treatment that resulted in 96.5 ± 1.3% DNA removal, while ECM components and mechanical properties were well maintained.
In the step of DNA removal, efficiency of DNA precipitation are influenced by NaCl and PEI concentrations.
