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RecA is involved in DNA recombination.
Homologous and site-specific DNA recombination has revolutionized genetic engineering.
Ten years ago, genome editing tools were limited to virus-driven integration and homologous DNA recombination.
These results indicate that the gene product has a DNA recombination activity.
New mutagenesis methods developed for random mutagenesis, focused mutagenesis and DNA recombination were surveyed.
The discovery of plant DNA recombination techniques triggered the development of a wide range of genetically modified crops.
Almost all cellular organisms employ RecA orthologues to guide the strand invasion reactions necessary for DNA recombination and repair.
Holliday junction resolution performed by a variety of structure-specific endonucleases is a key step in DNA recombination and repair.
Several positive selection system-based vectors have been considered efficient tools to date for simplifying in vitro DNA recombination procedures (Liu et al. 2011).
We have recently established split-Cre that allows DNA recombination to be controlled by coincidental activity of two promoters, thereby increasing spatial specificity of Cre-mediated DNA recombination.
These data suggest that integrases have high DNA recombination efficiencies in mammalian cells.
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