The median HBoV viral load was 2.61 log copies/ng of DNA (range 1.26 4.26 log copies/ng of DNA).
Assay performance was equivalent across the input DNA range (<2 ng (limit of detection) to 100ng dsDNA, 41.4ng median DNA input, p = 0.852, chi-squared test) (Fig. 4d,e,f).
We extracted on average 42.4 μg DNA (range 1.8 97.4 μg) out of 4.5 mL blood.
The mean value of parasite load was 9,502 parasites/µg tissue DNA with range of 2 to 144,586 parasites/µg tissue DNA (Table 3), while all leprosy lesions (n = 15) were negative.
In the DNA concentration range of 5−300 nM, a dramatic increase of FAM fluorescence intensity was observed, demonstrating that the SRM-DNA assembly approach is effective in probing biomolecular interactions.
Previously, a DNA content range of 2C = 0.2 1.8 pg was reported (Kapraun 2005).
The manufacturer reports a HBV DNA linear range of 20 IU/mL to 1.7 × 10 IU/mL.
While the overall 2C DNA content range of 0.2 2.8 pg is relatively wide, five of the orders (Gelidiales, Gigartinales, Gracilariales, Halymeniales and Rhodymeniales) have particularly narrow ranges of DNA contents [see Additional Information].
Extracts were sonicated to achieve a sheared DNA size range of ∼0.5 2.0 kb, and then centrifuged for 10 min at 13 000 r.p.m. at 4°C.
Copy number variants (CNVs) are deletions and duplications of stretches of DNA ranging from 1 kilobase to an entire chromosome.
