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The remaining portion of the original bloodstain extract was used to isolate DNA for DNA profiling using autosomal STRs in order to identify the donor of the bloodstain.
5 10 µl of DNA at 20 30 ng/µl for each sample were sent to DNA Labs, Sydney IVF (Sydney, Australia) and DNA profiling using the internationally recognized Identifiler system was performed, followed by short tandem repeat (STR) analysis.
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The BJAB cells used in these studies were DNA profiled using the Identifiler kit (Applied Biosystems) in January 2010.
When it was difficult to confirm the DNA profiles using these primers, a third primer, RAPD17 (5'-AACGCGCAAC-3') [ 30] was used to confirm the identification.
DNA profiles using two highly polymorphic microsatellite markers (D1S1586 and D3S1765) showed that six biliary tract carcinoma cell lines are unique and unrelated.
In addition, we also show that the quality of input DNA profile used for background normalization when calling peaks for a ChIP-seq dataset is critical.
Consequently, we performed DNA methylation profiling using DNA extracted from whole blood of monozygotic (MZ) twins discordant for breast cancer and the Infinium DNA methylation BeadChip technology covering more than 450 000 CpG sites genome wide (13, 14).
Consequently, we performed genome-wide DNA methylation profiling using DNA extracted from whole blood of MZ twins discordant for breast cancer.
While we assessed the validity of DNA pooling for DNA methylation profiling using the Sequenom EpiTYPER mass-spectrometry system, this method is potentially applicable to all bisulfite-based mapping techniques.
In summary, DNA methylation profiling using methodology compatible with degraded DNA has potential to be used as a diagnostic tool in improving the clinical decisions to differentiate recurrences from a second de novo tumour in FFPE samples.
Next, the isolated DNA was subjected to genome-wide level DNA methylation profiling using the Illumina HumanMethylation450K BeadChip.
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