Using the sensitive DNA profiling technique, LSSP-PCR (Low-Stringency single Specific Primer PCR), we previously showedthat hearts of BALB/c mice doubly infected with JG and Col1.7G2 T. cruzi populations were mainly colonized by JG strain, while Col1.7G2 was preponderant in the rectum and all other analyzed tissues [2].
DNA methylation is the most well-studied epigenetic alteration in cancer, owing in part to recent developments in genome-wide DNA methylation profiling techniques (10).
We used the DNA-based profiling technique T-RFLP and ordination-based statistical methods to compare fungal community compositions in surface soils from two remnants (located 1.6 km apart) and three sampling positions (beneath individual buloke canopies; grassy inter-canopy areas; and adjoining cleared paddocks).
But through a combination of cutting-edge techniques, including DNA profiling and GPS tracking of shipments, and the establishment of a high-level panel to adjudicate on evidence, the commission claims it will be able to demand those accused of corruption and smuggling appear before fact-finding hearings in The Hague.
"We covered all the scientific principles behind DNA profiling, including the specialist laboratory techniques and software used, as well as learning about the history and legal status of the process," recalls Phil.
Using case histories and interviews with practitioners, she follows the path of forensic investigations from the crime scene ("the scene is the silent witness", as the saying goes), through techniques such as DNA profiling (a bloodstain "a millionth the size of a grain of salt" is all that's needed nowadays), to the conclusion in the courtroom.
The technique, known as low copy DNA profiling, can capture the genetic profile of a suspect from samples so small they can be invisible to the naked eye — sometimes drawing DNA from a tiny number of human cells, according to Britain's Forensic Science Service.
Now there seems to be a widespread acceptance of most of these techniques, including the most powerful one, DNA profiling.
We propose that a high-throughput DNA pooling approach will facilitate the use of emerging methylomic profiling techniques in large samples.
