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Subsequently, captured cells are released at efficiencies of 86.1% by cleaving the DNA portion with a restriction enzyme.
Although scientists could sequence this gemisch, they would have to run the samples through their sequencing machines many times to zoom in on the human DNA portion, and it's not worth the cost.
(A sticky end is a short single-stranded DNA portion protruding from the end of double-stranded DNA [142]. A sticky end can combine with another sticky end only if their base sequences are complementary to each other, much like a key and lock fit together).
In the autogenous hypothesis, mitochondria were born by splitting off a portion of DNA from the nucleus of the eukaryotic cell at the time of divergence with the prokaryotes; this DNA portion would have been enclosed by membranes, which could not be crossed by proteins.
The biotinylated DNA product was then isolated by binding to streptavidin-agarose Dynabeads (Dynal), and digested with TaqαI (5'-T↓CGA), a 4-bp cutter that does not cleave the viral DNA portion of the biotinylated DNA.
The DNA portion of the primer consisted of either a random hexamer or a polyT region followed by a unique RNA sequence that labeled the 5' prime end of newly synthesized DNA molecule.
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Results of chromomycin banding, which stains GC-rich DNA portions, are highly variable within and between Tanacetum species and even among individuals in some cases.
Up to now, most studies using the ChIP assay have been focused on characterization of the DNA portions associated with the pulled-down ChIP material.
The amplified VH and VL DNA portions in the plasmids were sequenced by using a CEQ 2000XL-DNA Analysis System with Dye Terminator Cycle Sequencing Quick Start Kit (BECKMAN COULTER, Inc).
To achieve the necessary variation in cell structure and function, some DNA portions are "active," or expressed, in certain cells and at particular developmental stages, leading to the production of different end products.
For this to occur, various different possible scenarios could be hypothesized: inactivation of genes by deleterious mutations resulting in the accumulation of pseudogenes or loss of genome fragments by deletion of larger DNA portions via rearrangements [ 27].
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