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The type of genetic engineering done by Monsanto and its competitors, however, involves inserting genetic materials, sometimes from wholly different plant species and bacteria, directly into the DNA plants like corn or soybeans.
Genetic modification through transformation leads to an "event" that introduces the engineered DNA plants' genome.
Isolation of DNA was performed using Invisorb DNA plants 96 kit from Invitek (GmbH, Berlin, Germany) following kit's specific instructions.
DNA was extracted with the Invisorb DNA plants 96 kit from Invitek (GmbH, Berlin, Germany), according to the manufacturer's instructions.
'Polanka', 2C = 2.50 pg DNA) or maize (Zea mays 'CE-777, 2C = 5.43 pg DNA) plants (Doležel et al. 2007) were chopped together with the test samples with a new feather-edge razor blade, cutting pieces measuring 0.5 2 mm across (taking ∼60 90 s).
Different DNA extraction methods were used in needles: a modified protocol from Dellaporta et al. [ 78] for nSSRs, SAMPLs and SNP array D (detailed below); the commercial kit Invisorb DNA plants HTS 96 kit (Invitek GmbH, Berlin, Germany) for SNP arrays A and C (detailed below) and the commercial kit DNeasy Plant mini kit (Qiagen, Düsseldorf, Germany) for SNP array B (detailed below).
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Genomic DNA was extracted on plates using the Wizard® Magnetic 96 DNA Plant System (Promega) according to manufacturer's instructions.
The genomic DNA was extracted from leaves from the eight genotypes using the BioSprint DNA Plant Kit (Qiagen, CA, USA) according to the manufacturer's instructions.
Genomic DNA was extracted using MagAttract 96 DNA Plant Kit (Qiagen Inc., Valencia, CA) and KingFisher 96 (Thermo Electron Corporation, Waltham, MA).
This finding also suggests that the majority of undiscovered DNA plant virus diversity consists of variations on viral themes that have already been described.
The DNA extraction was performed on 100 mg of leaves using the DNA Plant Mini.
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