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extract DNA, PCR, and prepare PCR products for sequencing.
The student will help to sort botanical specimens as well as extract DNA, PCR, and prepare PCR products for sequencing.
To calculate the PCR amplification efficiency, a ten-fold serial dilution of template DNA (PCR amplified product) was performed and followed by qPCR (Supplementary Figure 1).
Single clones were genotyped with genomic DNA PCR and subsequently sequenced.
The KO genotype was verified via genomic DNA PCR analyses (Fig. 6b).
The qualitative SLG RT-PCR, semiquantitative DNA PCR, and viral antigen tests were compared.
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Libraries were made using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, USA).
WGS libraries were constructed using the TruSeq DNA PCR-Free sample preparation kit (Illumina, Inc., CA) following the manufacturer's instructions.
TruSeq DNA PCR-Free libraries were prepared from blood and FF tissues using 1 μg of input DNA according to the manufacturer's instructions (Illumina, San Diego, CA).
TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) were used to generate the sequencing libraries.
Sequencing libraries were generated using a TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) following the manufacturer's recommendations, and index codes were added.
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