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Additionally, associated anisotropy analysis of the TAMRA-aptamer reveals both quenched and unquenched states that experience significant coupling to the DNA motion.
Many of these approaches require DNA motion through nanopores to be slowed by several orders of magnitude from its native translocation velocity so that the translocation times for individual nucleotides fall within practical timescales for detection.
Features of DNA motion at the interface, such as evanescent-field residence time, linear velocity and frequency of adsorption/desorption events were measured to assess the relative affinities of the oligonucleotides for the surface.
A detailed understanding of the kinetics of DNA motion though nanometer-scale pores is important for the successful development of many of the proposed next-generation rapid DNA sequencing and analysis methods.
In previous work, we and others observed that when the DNA dwell time in the pore is substantial (>1 ms), DNA motion in α-hemolysin (α-HL) pores leads to nonexponential kinetics in the escape of DNA out of the pore.
He had asked the Texas judge for a stay of execution but, having heard nothing, was heading to Amarillo to argue the DNA motion in federal court.
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In addition, we demonstrate that the collective motion of lowly and moderately variable genes within the genome vehicle, which are often considered as noisy and insignificant, play an important role in the formation of the neutrophil attractor, perhaps indicating the non-instructive signaling of genes related to small-amplitude DNA motions [24] [25].
Although certain MD simulations showed that the long component in tens of picoseconds was mainly from local protein (or DNA) motions, our extensive studies both in experiments and in MD simulations showed that the long component is from water protein coupled motions, mainly driven by local water network relaxation.
A motor-based microprobe is proposed using a tubular microengine powered by bio-assembled enzyme as catalyst and exploited for washing-free detection of DNA through motion readout.
This makes DNA replication motion at least partly a stochastic process that is dependent on sequence properties such as length and base composition.
Because DNA loop motion is passive, this model is essentially a modified sliding scheme.
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