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The monophyly of Ditrysia is recovered in the maximum likelihood analyses based on DNA, morphological and combined data sets (Figs. 2, 3 and 4, Additional files 7, 8, 9, 11, 12, 13).
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It allowed us to perform double or triple labeling for immunofluorescence microscopy and thus to determine the sequence of phosphorylation on each site in the same cell and with exact knowledge of the stage of the cell cycle by monitoring DNA morphological changes and phosphorylation of Histone H3.
Using DNA barcode, morphological and life history data, the single species A. fulgerator was proposed to contain at least ten distinct phylotypes, which were suggested to be ten species.
Population structure and intraspecific diversification of Ae. tauschii were well characterized in our previous studies based on chloroplast and nuclear DNA and morphological variations [25] [28].
For example, in a contact zone between central European newts, Babik et al. [ 46] document a tighter correlation between nuclear DNA and morphological hybrid indices than between mtDNA and morphology.
Although the support values are the highest for the smallest data set including 268 taxa (with both DNA and morphological data available for all taxa), and weakest in the trees including all 473 taxa (with either or both types of data), we choose to present the latter (Figs. 2, 3 and 4).
Moazzeni et al. (2010) analysed the phylogeny of 28 Iranian taxa of Isatidae by using ITS sequence of ribosomal DNA and morphological characters.
Flies destined for DNA and morphological analysis were stored in 100% ethanol.
The microsatellite DNA and morphological analyses both were subject to summary ordination analyses.
It will take further DNA and morphological studies of all taxa within the group to determine which species are valid.
The tree of combined DNA and morphological data consists of five major clades labeled A-E with strong to maximum support, which are clustered together with maximum support (Fig. 3).
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