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The method is efficient to produce a large number of DNA markers based on EST database.
In the past two decades, it has become possible to construct high-density maps for almost all areas of chromosomes using DNA markers based on sequence polymorphisms.
DNA markers based on the sequences of such genes may be used by breeders for MAS in triticale, although the causal link between these genes and Al resistance remains to be established.
High-resolution mapping, using a combination of additional D15Mit markers, novel polymorphic DNA markers based on nucleotide repeat sequences and an additional cross to C57BL/6 (generating a second recombinant line), further narrowed the critical region to an interval of 1.1 Mb (36.4 37.5 Mb) flanked by heterozygous sequences (Fig. 2B).
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The studies were performed with respectively 120 and 80 male piglets that were tested for susceptibility or resistance towards ETEC O149 F4ac by a DNA marker based test.
Progress has been made in the development of DNA marker based genetic maps in globe artichoke [ 12- 14].
Generally, gene flow estimations using plastid DNA markers are based on DNA transmission through seeds in most angiosperm species [ 34].
This population has been genotyped with four nuclear DNA markers selected based on linkage to QTL for resistance to late blight and plant maturity for association analysis [ 55].
Finally, we examined the relationship between phenotype and genotype using chloroplast DNA markers developed based on the specific mutation of the Wogon-Sugi chloroplast genome.
Molecular markers based on DNA methylation, that is methylation markers, are of particular interest as recent studies indicate that DNA methylation can be easily detected in cervical scrapes using sensitive PCR-based methods like methylation-specific PCR (MSP).
In conclusion, by a genome-wide analysis, markers based on DNA methylation were identified for high accuracy of diagnosis of UCs using urine samples in our preliminary study.
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