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However, DNA marker analyses have revealed substantial genetic differences (Puri and Zhong 2010).
Cytogenetic and DNA marker analyses suggested that Group 1 and Group 2 have homomorphic and heteromorphic sex chromosome pairs (XY), respectively, and that the sex chromosome pairs of the two groups evolved from a common ancestral pair.
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This is not surprising in view of the large number of Asian, African and South American accessions without any polymorphisms for the DNA markers analysed.
The DNA markers and analyses used in species identification are identical to those used in taxonomic studies to identify relationships between different species.
The efficiency of a DNA marker system for analysing diversity relies upon the extent of polymorphism detected by uncovering a large number of markers spanning the whole genome (Luikart et al. 2003).
The B. oleracea mapping population (163 F2 plants), the evaluation of plant response to downy mildew, and the procedures for plant DNA extraction and molecular marker analyses have been previously described (Coelho and Monteiro 2003; Farinhó et al. 2004, 2007).
They are an important tool for many genetic applications, including mapping of quantitative trait loci (QTLs), comparative mapping, identifying DNA markers for fingerprinting, analyses of population genetics and phylogenetics, genome sequence assembly, relating physical and recombination distances along the genome, and map-based cloning of genes.
MJ and GA performed the genetic marker analyses, and did the DNA bank assembly.
We analysed a coat colour gene (KIT) to identify a DNA marker that could be useful for Cinta Senese meat product authentication.
M: DL2000 DNA marker.
M, DNA marker.
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