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The interactions of cisplatin with other anti-cancer agents on the DNA level have been studied extensively in pre-clinical experiments.
Conventional sequencing has also been used to detect mutations of LKB1 at mRNA level and some mutations missed by sequencing at the DNA level have been discovered by mRNA-based approaches (Abed et al, 2001).
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Whether this acceleration and compositional bias were caused by adaptive evolution at the AA level or directional mutation pressure at the DNA level has been vigorously debated.
The mechanism of action of these agents at the DNA level has been well described.
For example, although common variation at the DNA level has been densely and routinely explored for single SNP associations, interactions have been largely ignored.
During the last ten years, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in animal genetics studies.
The limited research on characterization at the DNA level has hindered the improvement of A. oxyphylla, and little DNA variation has been detected by inter-simple sequence repeat (ISSR) markers (Wang et al., 2012).
One month later during the course of therapy, the HBV DNA level had dropped to 200 ge/ml and another month later, HBV DNA was undetectable and remained undetectable till date.
However, the extreme repetition within the extender domains, which can be identical even at the DNA level, has caused difficulties in sequencing the ORFs (open reading frames) of some RTX adhesins.
Third, the serum Epstein-Barr virus (EBV) DNA level has been demonstrated to be an important prognostic factor among patients with recurrent/metastatic NPC [ 32]; however, we failed to include it in our analysis due to the lack of data at diagnosis in our cancer center (116 patients, 23.0%).
This phenomenon is actually a byproduct of the basic repeat unit (at the DNA level) having any length that is not a multiple of 3. We found that only for 18% of all TR-proteins (806 instances) was this phenomenon present.
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