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DNA was isolated from normal and endoreduplicated root tips of Allium cepa to determine the change in DNA level due to endoreduplication process induced within root tip cells (data published [ 25]).
The mechanism by which Helitrons acquire novel sequence remains largely unknown, but is hypothesized to occur at the DNA level due to transposition of both intron and exon sequences.
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CY treatment increases the concentration of extracellular DNA levels due to the large amount of cell death, so it seems logical to surmise that DNAse may reduce the local concentration of DNA in the spleen and thus, impair the autoantigen-mediated expansion of high affinity DNA-reactive B cells.
The variability in the expression potential of HERVs at the DNA level is due to defective nature of most HERV proviruses and to the existence of unfixed HERV proviruses that are not present in all human individuals [ 16, 54].
We cannot determine from the transcriptional sequence data if these isoforms are present at the genomic DNA level, or are due to alternative splicing.
After all, single case studies are not sufficient to identify global adaptive patterns in sequences at the DNA and protein level due to environmental temperature.
Each experiment utilized a different aliquot of cryopreserved cells from the individuals examined, though the aliquots were all taken from 5th passage cells to control for cell expansion dynamics, including any potential alterations in DNA methylation levels due to growth.
In acidified mesophilic maize-silage-fed fermenters, sequences affiliating with the hydrogenotrophic order Methanomicrobiales were abundant at the DNA level [31, 46], most probably due to their stability in acidic environments, but they are not necessarily the most active at such conditions (see below).
Quantities of human 18S rRNA gene copies by qPCR were relatively constant showing consistent loading of human cells on the swabs with good stability of DNA and fluctuations in DNA levels were not due to sample quality (Figure 1).
Due to the lack of polymorphism at the DNA level, the crop has not been subject to marker-assisted selection or resistance gene cloning.
DNA-fingerprint analysis revealed changes at the DNA level.
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