Sentence examples for dna lacking the from inspiring English sources

Bacterial host restriction endonucleases (REase) attack invading foreign DNA lacking the imprinted modification pattern characteristic of the host DNA [4].

Finally, we measured the sensitivity of our mutation detection assay by performing a dilution of mutation template into increasing amounts of wild-type genomic DNA lacking the mutation.

In contrast, barely any GFP positive cells showed up in the mock transfection controls using empty vector DNA lacking the Sox2-TALE-TF.

Our model experiment showed that single DNA molecules carrying a full-length insertion into a 6-kb target can be recovered by Multiplex PCR of target amplicons followed by Hybridization Enrichment (MP-HE), even in the presence of a huge excess of genomic DNA lacking the insertion.

If long stretches of DNA lack the necessary identity by descent to function as replicators, then sexual organisms certainly lack it.

The fact that partial methylation was attained in the male germ line (Figure 5B) but it was not observed in the soma (Figure 4A and B) demonstrates that the (ChβGI 2 DNA lacks the potential to maintain methylation in the paternal allele.

However, they are quite different in sequence and three-dimensional structure since DNA lacks the 2′ hydroxyl group of the RNA.

Plasmid pBSX1cTIM, containing the wild-type gene of cTIM, and E. coli strain DF502 (strepR, tpi–, and his–), whose DNA lacks the gene for TIM, were generous gifts from N. Sampson.

It is worth noting that due to the 4qA-specific SNPs residing at the 3' end of the 4qA BS PCR oligonucleotide primers, multiple rounds of primer freeze-thaw, which leads to partial primer degradation, results in a loss of specificity and a consequent amplification of minor products from genomic DNAs lacking the 4qA allele (Additional file 1: Figure S1B).

The resulting Δ cpaAIR gene disruption mutant should not produce a functional CpaAI Type II restriction endonuclease and, therefore, should be efficiently transformed with plasmid DNA lacking methylation by the FnuDIIM methyltransferase (5′-m5CGCG-3′) [ 15].

Rather, all PCNA loaded onto P/T DNA by RFC is unloaded back into solution by RFC.> The experiments depicted in Figure 4 were also carried out on a Cy3-P/T DNA substrate lacking the single-stranded DNA flap (Cy3-P/T DNA-No Flap, Table 1) and yielded the same results.