While transcription occurred in the hydrogel nuclei where DNA was localized, these results indicate that translation was likely not localized to the cell-mimic a given mRNA originated from.
At all time points (1 h–120 h) DNA was localized in vesicles and after 96 h the vesicles containing plasmid DNA were localized around nuclei (Figure 3F and 3I).
Mitochondrial DNA was localized in fixed cells in two ways.
Parvoviral DNA was localized to glomerular parietal cells and visceral epithelial cells using in situ hybridization.
Our own study [ 20] also found that the DNA was localized in the cytosol and was not accessible to intracellular TLRs in the endosomes.
For clonal amplification, DNA was localized to Ion Sphere particles (LT), which were automatically enriched with the Ion OneTouch ES system (LT).
Parvovirus DNA was present in kidney tissue [ 22– 24], although the presence of viral genome in circulating immune cells cannot be excluded as a source of this signal and DNA was localized to glomerular endothelial cells [ 20] but not to visceral epithelial cells.
In addition, previous studies have shown that AtCDT1a, a component of the prereplication complex for DNA replication, is localized in both the nucleus and chloroplasts, and is involved in the regulation of endoreduplication and chloroplast division [ 33, 34].
At first, since fusion of telomeres leads to the formation of dicentric chromosomes and chromosome breaks, it seemed paradoxical that Ku70/80, a protein that facilitates the joining of DNA ends, is localized at telomeres.
As the T-DNA insertion is localized downstream of the Stop codon in the lif2-4 mutand and its phenotype is similar to wild type, it is likely that LIF2 protein is produced.
