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Considering the effects of electrodes, the relative conductance of Ag-doped DNA is calculated byI V curve and is the average of many points on DNA for each Ag+concentration (The relative conductance is the ratio of the conductance of Ag-doped DNA ropes to the conductance of the loop which was composed of tip, gold electrode, and inner circuitry of AFM).
The percentage of methylated DNA is calculated as described previously [ 20, 68].
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The concentration of fungal DNA was calculated by comparing a dilution series with defined amounts of DNA of lambda phage (methylated, from Escherichia coli host strain W3110).
Based on the CV and spectroscopic results, the binding constant between NTZ and DNA was calculated to be 4.1 × 105 M−1 and 5.0 × 105 M−1, respectively.
The average ratio of mitochondrial to nuclear DNA was calculated from the results of triplicated reactions in each experiment, and the values were normalized to that of non-treated cells.
The binding constant (K) between LPT and DNA was calculated as 6.03 × 105 M−1, 4.20 × 105 M−1 and 3.50 × 104 M−1 for electrochemical, UV vis spectrophotometric and fluorescence spectroscopic techniques, respectively.
In pH 4.0 phosphate buffer solution (PBS), the binding ratio between STS and salmon sperm DNA was calculated to be 1 1 and the binding constant was 1.67 × 104 L/mol.
The percentage of BamHI and EcoRI digestible PCR products among total DNA was calculated after quantifying DNA bands using Image J. pCEP4-Flex1 or pCEP4-Luc plasmids contain the Epstein-Barr virus (EBV) replication origins and propagate as episomes in mammalian cells10,67.
Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G + C /(A + T) of DNA was calculated as about 0.77 for various DNA samples.
The amount of DNA was calculated according to the linear calibration curve of DNA (F = 2 × 10−4 C + 0.0437 R = 0.999 4).
The mass fraction of phosphorus (P %) in plasmid DNA was calculated to be 10.22 %, using the equation P % = 2×bp × 30.974/MDNA, Where bp is the number of base pairs in plasmid molecules, and MDNA is the molecular weight of the plasmids.
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