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In the second phase (10 50 ns), DNA is accommodated into the binding cleft by adjusting its groove width and orientation.
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In the Dpo4 models generated herein, using the BPmi3 orientation the pyrene moiety of [+ta]-B[a]P-N2-dG points toward the duplex region of the DNA, and is accommodated without distortions in AS1, but with distortions in AS2.
Structurally, 1, N-εdG adopts the anti conformation about the glycosyl bond and is accommodated within the DNA helix.
The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice.
How could such deficits in DNA content be accommodated within the nucleosome?
An important conclusion from the crystal structures was that uracil within the helical context of DNA could not be accommodated within the buried uracil-binding pocket.
The maximum amount of genomic DNA that could be accommodated by the WGA kit (20 ng) was amplified and then re-amplified using the GenomePlex WGA re-amplification kit following the manufacturer's instructions.
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A mechanistic understanding of how different W C and non-W C structures are accommodated durinon-W Creplication requirestructuresthe structure are properties of the traccommodatedte (TS) for the rate-duringining step (RDNA in the DNA polymereplicationactive-site.
Pol η-mediated TLS preserves ongoing DNA replication by incorporating nucleotides at lesions that cannot be accommodated by the replicative DNA polymerase complex (Sale et al. 2012).
The orientations of the R,R- and S, S-DHB-dA adducts in the major groove of DNA with respect to the bis-alkylated adenine nucleobase allows for thymine in the complementary strand to be accommodated in the DNA duplex with relatively little distortion.
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