As mentioned, on small length scales DNA is a stiff polymer, while on large length scales it adapts a random coil conformation.
Large-scale sequencing of DNA is a new art that the consortium learned by doing it.
DNA-PKcs is a large PI3-kinase-related protein kinase (PIKK) that plays a central role in DNA double-strand break (DSB) repair via nonhomologous end joining.
The data indicate that the binding of the oxidized SOD1 to DNA is an exothermic reaction with a large negative enthalpy change of –27.65±0.45 kcal/mol and a binding equilibrium constant of ∼100 nM, revealing that the strong binding between the oxidized SOD1 proteins and ssDNA is mainly determined by the electrostatic interactions.
AP2-ERF TF containing highly conserved AP2/ERF DNA-binding domain, is a large family unique in plant.
The E. coli DNA polymerase III holoenzyme (DNA Pol III HE) is a large macromolecular machine that contains 10 proteins (α, β, ɛ, θ, τ, γ, δ, δ', χ, and ψ) with a combined molecular weight close to one megadalton (Johnson and O'Donnell, 2005).
Frog virus 3 (FV3) is a large DNA virus that encodes ∼ 100 proteins.
HSV-1, like vaccinia, is a large DNA virus, which can be easily manipulated to allow tumour selective virus replication (MacLean et al, 1991; Martuza et al, 1991; Rampling et al, 2000) and allows insertion of therapeutic genes of up to 30 kb (Roizman and Jenkins, 1985).
pRM13516 does not appear to be related to any previously reported EHEC or STEC plasmids, rather, there is a large DNA segment containing type IVb pilus genes and virB1- virB11 that are also present on Escherichia coli plasmids pChi7122-3 and pR721 and Salmonella plasmid pSH146-65 (Additional file 1: Figure S10B).
KSHV is a large DNA virus that encodes over 80 different proteins and is the causative agent of several diseases including not only KS but also the hyperproliferative B cell disorders, primary effusion lymphoma (PEL) and multicentric Castleman's disease [ 71].
