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They were identified based on the nuclear ribosomal DNA internal transcribed spacer 1 (ITS1) region using a combination of Single Strand Conformation Polymorphism (SSCP) and DNA sequencing [44], [60].
Identifications were supported by using mitochondrial DNA cytochrome oxidase I (COI) and nuclear DNA Internal transcribed spacer 2 (ITsequencesncesequences
In this study, P. oligandrum isolates were isolated from New Zealand mushroom composts, and their ribosomal DNA internal transcribed spacer (ITS) regions were amplified using the polymerase chain reaction (PCR).
Two sets of primers, one newly designed and targeting the nuclear ribosomal DNA internal transcribed spacers (ITS) and the other amplifying a fragment of mitochondrial large subunit ribosomal DNA met the requirements of high specificity and sensitivity, amplifying DNA from a broad range of larger basidiomycetes, with no amplification of plant, bacterial or ascomycete DNA.
The aim of this study was to develop polymerase chain reaction (PCR) assays for the diagnosis and early detection of latent infections of blueberry stems by Botryosphaeria spp. Species-specific primers, based on the ribosomal DNA internal transcribed spacer region and β-tubulin gene, were designed and selected for use in PCR assays.
Phylogenetic analysis using the sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) loci was not conclusive but seems to show that C. bulbifera and C. virosa are monophyletic, while C. douglasii may not be.
Similar(31)
"Credibility is external - DNA is internal.
Telomeric DNA and internal control are indicated by arrows.
Individuals were initially identified to species and genotyped using the ribosomal DNA second internal transcribed spacer.
We therefore investigated whether HIV-1 IN could cleave similarly longer DNA at internal positions.
To allow for comparison of sense and antisense levels, all samples were normalized to a radiolabeled DNA oligonucleotide (internal control) loaded alongside the samples on the gel [e.g. ((sense or antisense)/internal control)/(U6/internal control)].
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