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These slipping events occurred in one fast rip, not in several steps, indicating that a single motor– DNA interaction is disrupted in each case.
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The most important compounds, whose effect is based on DNA interaction, are anthracyclines, cytotoxic antibiotics, and platinum-based cytostatics [ 18– 218.
Ethidium bromide assay for the lipid:DNA interactions is consistent with the general observation that significant lipid:DNA interactions do not guarantee on improved transfection efficiency cationic lipid mediated gene delivery.
Briefly, the specificity of the protein - DNA interactions is unsuitable to regulate the genetic network at that high level what the collected experimental data suggests.
The nucleosome-DNA interaction is in thermodynamic equilibrium.
This affinity, but not the specificity of this protein-DNA interaction is affected by the aminoacids of the homeodomain N-terminal arm [ 18].
While the identification of p63 bound cis-regulatory elements in keratinocytes has received much attention, the mechanics of p63-DNA interaction is still relatively unknown.
In this paper, the term "protein-DNA interaction" is meant to denote the binding of one TF to the upstream regulatory DNA element of another NR gene; that could either activate or repress its expression.
The compound bulge DNA interactions were characterized via UV melting experiments, circular dichroism and were quantitated by surface plasmon resonance with Kd of 41.5 μM.
In this report, we find that DNA interactions are not affected by the presence of positive H3 methyl modifications.
Protein:DNA interactions were detected by two commonly used assays based on the LacZ reporter: X-gal colony-lift filter assay and ortho-nitrophenyl-galactoside (ONPG) liquid assay.
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