This reinforces the idea that these (erythroid specific) transcription factors, probably in complex with other proteins, are mainly responsible for the stability of the DNA interactions between regulatory elements of the β-globin locus.
However, if such long-range contacts are dynamic just like the DNA interactions between cis-regulatory elements within a locus [24], [46], they are expected to be disrupted during the time of α-amanitin treatment.
Condensin most likely acts by establishing DNA interactions between its binding sites on DNA strands that happen to come into proximity (D'Ambrosio et al. 2008; Haeusler et al. 2008; Thadani et al. 2012).
In a contrasting model, condensin has been proposed to act by providing DNA interactions between its chromosomal binding sites in a more stochastic manner, without the need to engage into higher order assemblies.
To determine a specific protein-DNA interaction between SALL4 and the ABCA3 promoter, nuclear extracts from HeLa cells overexpressing SALL4B (Figure 4B) were used in EMSA assay.
There is also a protein-DNA interaction between Nrg1p and Ykl177W, a dubious open reading frame.
The fourth nickel toxicity modulating network includes the protein-DNA interaction between Nrg1 and Coy1 (Ykl179C) (figure 2d).
The protein-DNA interaction between TCP transcription factors and SlTCP12,-15 SlTCP12,-15moter elements suggest thandTCPs regulate the expression of other members of their own family.
The first approach is based on the explicit detection of the physical protein-DNA interaction between regulators and promoters of target genes.
Thus, in the absence of any observable alterations to the affinity, geometry, or stoichiometry of the protein-DNA interaction between Orc1-1 anDNANA, we propose that the differential sensitivity to trypsin reflects a conformational alteration of the ATP-bound form of the protein on DNA that renders it permissive to recruitment of the MCM helicase.
