Exact(8)
They used a genetic tool and PCR-based approach to amplify a tiny region of the genome in dozens of mice, some exposed to ECT-like electrical stimulation and some not, to compare the status of cytosine at similar stretches of DNA in brain tissue.
PCR was also used to detect Acanthamoeba DNA in brain tissue.
Diagnosis was made by identification of amebas in brain sections by immunofluorescence analysis and confirmed by demonstrating Acanthamoeba mitochondrial 16S rRNA gene DNA in brain tissue sections.
In conclusion, Acanthamoeba infection was demonstrated by hematoxylin and eosin and immunostaining of brain tissue and PCR detection of ameba mitochondrial DNA in brain tissue.
Both transplant recipients died, and a postmortem diagnosis was determined by immunohistochemistry and real-time PCR detection of B. mandrillaris amebae DNA in brain biopsy specimens (35 ).
PCR has been used to identify ameba DNA in brain tissue and CSF of persons suspected of having balamuthiasis (2 ) and in brain tissue of a patient with primary amebic meningoencephalitis caused by N. fowleri (11 ).
Similar(52)
Interestingly, HSV-1 DNA in brains of elderly normal as well as AD patients, may not preclude a role for the virus in the disease, instead the presence of this viral DNA in the brains of apolipoprotein E4 carriers is associated with AD20,21,22.
Of note, bacterial studies including Gram staining and PCR for bacterial DNA in brains were inconclusive.
To measure SIV DNA levels in brain, genomic DNA was isolated from 50 mg of tissue (basal ganglia) using the DNeasy Kit Quiagen.
In summary, this study analyzed mitochondrial DNA variation in brain and found increased mitochondrial DNA substitutions in subjects with SZ compared to controls similar to findings in blood [58] for SZ.
32 In particular, clinical evidence has shown clonal DNA mutations in brain tumours, 33 34 35 36 implying that DNA damage could be the initiating event.
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