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In summary, each Cy5-labelled cDNA generated from an RNA sample was co-hybridised with Cy3-labelled DNA generated from M. tuberculosis H37Rv genomic DNA.
To a background of DNA generated from natural outdoor aerosols, known quantities of rRNA gene copies from distinct organisms were added producing corresponding hybridization intensity scores that correlated well with their concentrations (r = 0.917).
Herein, a new CL strategy has been developed for the sensitive, convenient and direct monitoring of the cleavage of ssDNA by specific S1 nuclease based on quite different CL response of AuNPs to long ssDNA and fragmented DNA generated from the enzymatic cleavage reaction of DNA.
Here, a lethal lesion is defined as the local modification of DNA generated from the direct and indirect action of ionisation radiation (i.e. a double-strand break).
DNA generated from wild type mice, appeared as short linear molecules of length 96±5 nm (S.D.; n = 100; Figure 2A).
RT-PCR amplification of endogenous gene transcripts used DNA generated from 50 200 ng of input RNA rather than genomic DNA.
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After chilling on ice for a further 2 min, the complementary DNA (cDNA) generated from the reverse transcription was stored at −20°C until ready for use.
Under this hypothesis, it would seem logical that a similar bias should exist in DNA sequences generated from unamplified DNA using the GS20.
DNA probes generated from mouse genomic DNA were hybridised and washed at 50°C.
This population of smaller-sized DNA species, generated from a single DNA sample, is referred to as a "library".
RNA was purified by pegGOLD (peqLab, Erlangen, Germany) using standard protocols, and complementary DNA was generated from 1 mg RNA using a complementary DNA synthesis kit (Roche, Basel, Switzerland).
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