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DNA gel extraction and plasmid preparation kits were purchased from Omega Bio-Tek.
Stable transformants were further analysed by PCR and DNA gel blot analyses.
PCR products were analyzed on 1% agarose gels and visualized using SYBR safe DNA gel stain (Thermo Fisher Scientific).
To improve the resolution, a novel two-dimensional DNA gel electrophoresis (2-DGE) method was designed.
PCR products were gel extracted using the Zymoclean DNA Gel Recovery kit (Zymo Research) and prepared for qPCR using the DNA Clean and Concentrator kit (Zymo Research).
The PCR products were separated on a 10% polyacrylamide gel, stained with SyberSafe DNA gel stain (Invitrogen Life Technologies) and imaged on a BioRad ChemicDoc System.
We acknowledge H. Kwon Lee for his contribution to the polyacrylamide DNA gel and assistance with data analysis of the fibroblasts cell adhesion studies.
PCR products were separated by agarose gel electrophoresis, stained with Sybr Safe DNA Gel Stain (Invitrogen) and visualised with a Gel Doc XR transilluminator (Bio-Rad).
PCR products were gel purified using AxyPrep DNA Gel Extraction Kit (Axygen) and cloned into pCRII-TOPO cloning vector (Invitrogen) according to manufacturer's instructions.
On the basis of designing DNA gel particles for therapeutic purposes, recent studies on the determination of the surface hydrophobicity and the hemolytic and the cytotoxic assessments of the obtained DNA gel particles have been also reported.
Quantum Prep™ Freeze 'N Squeeze DNA Gel Extraction Spin Columns were from Bio-Rad, USA.
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