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Immunoglobulin heavy chain polymerase chain reaction and sequencing were performed on 16 of 18 cases, which had adequate DNA for further analysis.
Thus 100 1 was selected as the optimal mass ratio of NPs to DNA for further study.
Because the glabrous branches of G. pacifica contain considerable mucilage, it is often difficult to obtain pure DNA for further PCR reactions.
This enrichment method is included in QIAGEN's QIAamp DNA Microbiome Kit and the QIAamp FAST DNA Stool Mini Kit, which isolate microbial DNA for further analysis.
PCR was performed as described above with faecal DNA initially subjected to universal bacterial PCR [12] to confirm the suitability of the DNA for further analysis.
PCR was performed as described above with biopsy DNA initially subjected to universal bacterial PCR [12] to confirm the suitability of the DNA for further analysis.
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[Editors' note: further revisions were requested prior to acceptance, as described below.] Thank you for resubmitting your work entitled "Assessing long-distance RNA sequence connectivity via RNA-templated DNA-DNA ligation" for further consideration at eLife.
To monetize, the game allows users to purchase extra DNA points for further customizing their creatures.
The MacClade program [38] was used to edit the DNA alignments for further analysis.
Putative exconjugants were purified twice on the same medium, before genomic and plasmid DNA extraction for further analysis.
The RT-PCR products were purified with the QIAquick PCR purification kit and were served as randomized DNA libraries for further functional selection.
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