Sentence examples for dna expression vector from inspiring English sources

After establishing our minicircle DNA expression vector, we tested whether AR protein delivered via minicircle DNA could be expressed for an extended period of time.

The developed cell line (CHOP-off-Bcl-xL), which constitutively expresses PyLT and inducibly expresses Bcl-xL, is capable of episomal replication with the use of the DNA expression vector encoding PyOri, EBNA-1, and OriP (pWP-Ang-EBNA/OriP-PyOri) and it is apoptosis-resistant.

We developed two computer-driven methods for improving epitope-driven HIV vaccines: the Epi-Assembler, which derives representative or "immunogenic consensus sequence" (ICS) epitopes from multiple viral variants, and VaccineCAD, which reduces junctional immunogenicity when epitopes are aligned in a string-of-beads format for insertion in a DNA expression vector.

An added advantage of transient in vivo DNA transfection is that the DNA expression vector is extrachromosomal, therefore, the expression of a targeted gene on the DNA vector would not be affected by the genetic background of genomic DNA.

A DNA expression vector bCN/BChE [ 14] was digested with XhoI to remove the BChE insert.

Three F0 founder mice, two females and one male, were generated by pronuclear microinjection with the in vivo DNA expression vector, bCN/BChE/hSA.

Two polyethylene oxide-based delivery systems comprised of reacting PEG polymers were designed for the delivery of DNA expression vectors.

In this report, we show efficient transfer of DNA expression vectors and siRNA oligonucleotides into a variety of primary cell types from different species utilizing the Nucleofector technology, including human B-CLL cells, human CD34+ cells, human lymphocytes, rat cardiomyocytes, human, porcine, and bovine chondrocytes, and rat neurons.

We have put these ideas into practice in two recent studies in which we generated enhanced cytotoxic T lymphocyte (CTL) responses, while retaining robust humoral responses, to wild-type viral proteins by immunizing mice with genetically modified forms of HIV-1 Env, Gag and Pol delivered in the form of plasmid DNA expression vectors.

CMV-HA tagged ubiquitin, CUL1, CKS1, and Flag tagged CUL7 DNA expression vectors were gifts from Drs. M. Pagano and Z. Q. Pan.

To confirm that Gag was poorly expressed from non-optimized transcripts transcribed in the nucleus, we transfected Jurkat cells with DNA expression vectors encoding optimized or nonoptimized gag sequences under control of the CMV promoter.