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Following [8], we approximated the DNA diffusion coefficient as D = kBT /6πηrg, where η is the buffer viscosity, rg is the gyration radius of the DNA molecule, and kB is the Boltzmann constant, which was 3.12 to 3.43 × 10−1 μm2/s in the temperature range of 25°C to 55°C, as shown in Figure 6b.
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Figure 6 DNA molecule mobility and diffusion coefficient distribution.
Atomic force microscopy (AFM) has also been used to observe the 1D sliding of the σ RNAP on the DNA and reported the 1D diffusion coefficient of 1.1 × 10 nm/s.
11 The rapid diffusion of β2 on dsDNA was confirmed by the visualization of individual clamps moving on phage λ DNA (48.5 kb) with a diffusion coefficient D=(0.134±0.009) μm s−1 (in 0.1 m NaCl; see Figure S3), thus indicating that β2 would travel the 26 bp effective diffusion length of the 36 bp dsDNA molecules in 0.3 ms (faster than our time resolution of 30 ms).
Electrophoretic mobility and the translational diffusion coefficient of the DNA molecules were obtained and compared with those of existing data. .
In particular, we show that using our comprehensive parameter estimation, our model displays similar behaviour as in (Elf et al., 2007), i.e. residence time of tR=5 ms, actual sliding lengths of slobs=90 bp, the relative time the molecules stays bound to the DNA of f=0.9 and the 1D diffusion coefficient of 0.046 μm s−1.
From the studies of the interaction in solution, the diffusion coefficient of free and DNA-bound NR, binding constant and binding site size of the DNA NR complex was also obtained simultaneously by non-linear fitting analysis of voltammetric data.
The diffusion coefficient of the drug-DNA adduct (1.02 × 10−6 cm2 s−1) is lower than that for the free drug (1.31 × 10−6 cm2 s−1) indicating slow diffusion of the comparatively high molecular weight drug towards the electrode in the cyclic voltammetric study.
Single-particle tracking of single-layered rectangular DNA origami sheets on SLBs previously resulted in a diffusion coefficient of D = 0.7 μm/s.
(a) DNA electrophoresis velocity versus electric field and (b) relationship of diffusion coefficient and buffer solution temperatures [11 13].
The convective-diffusive transport of DNA is analyzed using Peclet number described by experimentally measured electrophoretic mobility and the diffusion coefficient.
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