Since the animals are frequently infected latently with no or low seropositivity, a DNA based diagnostic method would be useful for surveys and detection of these viruses.
Meanwhile, we recently evaluated a DNA-based diagnostic method by the polymerase chain reaction (PCR) targeting the small subunit ribosomal RNA (SSU rRNA) genes of all four species of human malaria as reported (8 ).
Recently, DNA based diagnostic methods are being used for the identification of the pathogens.
Similarly to the MTIST, the miniature cyclone or multi-vial cyclone, was developed to simplify handling of the sample by sampling directly into an Eppendorf tube for easy application of immunological or DNA-based diagnostic methods rather than microscopy.
Little and Stevenson [10] demonstrated that search algorithms can be successfully used on unaligned nucleotide sequence data, the most accurate and precise algorithms were, respectively, the commonly used local alignment search tool, BLAST [25] and a diagnostic method, DNA-BAR [26], [27].
Indeed, up to 90% of women with CIN are HPV DNA positive depending on the diagnostic method used (Kiviat et al, 1992; Schiffman, 1992).
Armed with intellectual property protection on both compositions of matter (claims on isolated DNA molecules) and diagnostic methods, Myriad developed its BRACAnalysis® test [ 28].
yongonense strains, showing a sensitivity of 100 fg of genomic DNA, suggesting its feasibility as a diagnostic method for M. yongonense strains.
We also used the custom CGH array as a second-line diagnostic method to test DNA samples from 83 patients (or relatives when not available) with phenotypical data very suggestive of a disease and for whom only one point mutation or no point mutation was detected (18 Rett – typical or atypical – syndrome or neonatal encephalopathy, 42 CF or CFTR-related disorder, 23 severe hemophilia A).
Two bacterial detection methods were used: (1) standard microbiological culturing, and (2) polymerase chain reaction (PCR) based diagnostic method to detect bacterial genomic DNA.
