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PCRs were carried out in 10 μl total volume containing 1 U LongAmp Taq DNA polymerase, 1× LongAmp Taq reaction buffer, 300 μM of each dNTP, 0.4 μM of each primer, and 10 100 ng genomic DNA depending on DNA quality.
A peak at the 2 1 complex was observed in all cases, with an additional peak corresponding to either unbound PhoP or WB6 DNA depending on which one was in excess.
In such a situation, toxin subtype identification or strain characterization would be difficult, but perhaps possible, using traditional DNA-based methods [ 19, 21] as clarified samples can contain small amounts of DNA depending on the degree of purity.
However, cyanoacrylate fuming significantly effects the amplification of DNA depending on the extraction methods.
A new study reveals that sperm carry different chemical tags on their DNA depending on whether their owner is lean or obese.
For example, AuNPs can strongly adsorb thiol-capped DNA, depending on their large specific surface area and high surface free energy, and AuNPs with a diameter larger than 30 nm are more stabilized and essential for many diagnostic applications [20, 24].
Similar(41)
The binding of a TF to DNA depends on many factors, which will vary from one context to another.
Many important molecules, including DNA, depend on hydrogen bonds for their structure.
Structure and maintenance of genomic DNA depend on the activity of these enzymes, and without them DNA replication and cell division are impossible.
The study of the behavior of these proteins provide information about the function of chromosomes, since part of the control of the genetic information encoded by DNA depends on interactions with proteins.
Interactions between proteins and their ligands, such as small molecules, other proteins, and DNA, depend on specific interatomic interactions that can be classified on the basis of atom type and distance and angle constraints.
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CEO of Professional Science Editing for Scientists @ prosciediting.com