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Plasmid integration into MiaPaCa2 cells was confirmed by PCR amplification of genomic DNA (data not shown).
PCR analysis revealed the recombination in the recombinant BAC DNA (data not shown).
Both knock-out cells stimulated normal induction of ISGs in response to transfected virus-derived DNA (data not shown).
Notably, similar EMSA results were obtained when the modified τPro and τMTBD were incubated with DNA (data not shown).
We also found no evidence of promoter methylation of these genes in LCL genomic DNA (data not shown).
In addition, many interphase cells had deformed nuclei and the presence of extranuclear DNA (data not shown).
In all cases investigated, the changes that had been introduced were maintained in the replicated DNA (data not shown).
Immunoprecipitation reactions using preimmuno serum show very little amplification of the SALL4 promoter in the immunoprecipitated DNA (data not shown).
Gene-specific primers for these selected genes resulted in comparable amplification efficiency as shown by amplification of genomic DNA (data not shown).
These matrices did not bind GFP-PlyGBD and were DNase-insensitive, thus they did not consist of the B. anthracis exopolysaccharide or DNA (data not shown).
The 19 other inserts sequenced contained other cloned sections of microbial community DNA (partial copies of bacterial DNA encoding polymerase genes and other bacterial DNA; data not shown).
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