We obtained PCR-amplified DNA corresponding to these genes from the DNA inventory of San Raffaele Hospital; all DNA samples had previously been sequenced by standard Sanger technique.
Primer pairs that would result in PCR products ranging from 180 to 300 bp were designed to amplify the DNA corresponding to the above sites, and were used to measure the immunoprecipitated DNA fragments by PCR.
Dr. Bork and his colleagues searched for fragments of DNA corresponding to the genomes of 1,511 different species of bacteria.
In these experiments, chips covered with human DNA corresponding to various genes were coated with both human and chimp RNA.
The sensitivity achieved was 7 pg of lupin DNA, corresponding to a percentage of less than 0.1% of lupin flour in the foods.
Multiplex RT-PCR utilizing both orthopox- and alphavirus-generic primers yielded amplification of DNA corresponding to the expected sizes of the orthopoxvirus and alphavirus fragments, respectively.
Gene-specific primers (EHa-FwA and EHa-RvI) were designed from the DNA sequences of the 5' and 3' RACE products, and subjected to PCR to amplify the DNA corresponding to the open reading frame of target gene.
DNA corresponding to the P-B mRNA was amplified by PCR using cDNAs from various bovine tissues including tooth germ, submaxillary gland, parotid gland, lachrymal gland, heart, liver, stomach, pancreas, spleen, kidney, adrenal, and ovary.
The detection limit was 60 fg DNA (corresponding to 19 cells) with pure cultures and 4 × 104 CFU equivalents g−1 lyophilized sample consisting of mixture of rhizosphere soil and roots.
The detection limits of the cox1 real-time PCR assays for cestodes and nematodes were 10 copies/reaction of extracted DNA, corresponding to CT values of 33 and 31 respectively.
This suggests that coils can be added or removed by duplication or deletion of the DNA corresponding to one or more coils and explains how homologous proteins can have different numbers of coils.
