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Cell DNA content was quantified by flow cytometry in permeabilized, propidium iodide-stained cells [22], [65].
The proportion of each of these cell types, in addition to any other cells with unusual DNA content was quantified during the course of RNAi induction (Fig. 8).
Nuclear DNA content was quantified by image analysis and nuclei were grouped into the following five classes: hypodiploid (DNA content ≤1.49 C), diploid (DNA content between 1.5 C and 2.39 C), hyperdiploid (DNA content between 2.4 C and 3.59 C), tetraploid (DNA content between 3.6C and 5.1C) and hypertetraploid (DNA content >5.1C) (Table 1).
DNA content was quantified using a FACSCAN (Becton Dickson).
To analyze the cell cycle progression of TT cells, the DNA content was quantified via flow cytometry.
DNA content was quantified with BioTek Plate reader spectrophotometry software (BioTek, Winooski, VT, USA) at 570 nm.
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Collagen, GAG, and DNA contents were quantified in engineered cartilage.
The relative mitochondrial DNA (mtDNA) content was quantified by qRT-PCR using primers for Cytochrome b (mitochondrial genomic DNA) and nuclear beta Actin (for nuclear genomic DNA).
Mitochondrial DNA (mtDNA) content was quantified by competitive PCR according to the method described in detail by Willey et al. [ 7].
Protein content was quantified using EZQuant (Invitrogen).
Total protein content was quantified (NanoDrop).
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