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Copy number detection was measured by serial dilutions of known amounts of DNA containing the target sequences, either as genomic or plasmid DNA.
A gene, they argued, was a specific stretch of DNA containing the instructions to make a protein molecule.
Plasmid DNA containing the respective target DNA fragment was used as DNA standard in qPCR.
Briefly, a 9 kb mouse DNA containing the CD146 gene was cloned into the pL253 vector.
Samples of DNA containing the length-variant STR alleles are typically separated and genotyped by comparison to an allelic ladder.
Regarding PhaR-DNA binding, Figure 2A shows that PhaR mainly bound to the DNA containing the phaP promoter region (curve a), and barely bound to the DNA containing the phaR promoter region (curve b) or to the control DNA (curve c).
Moreover, the dissociation rate constant for the DNA containing the phaP promoter region (k off = 1.7 ± 0.4 × 10-3 s-1) was not significantly different from the dissociation rate constant for the DNA containing the phaR promoter region (k off = 0.9 × 10-3 s-1) or that of the control DNA (k off = 0.7 × 10-3 s-1).
PhaR mainly bound to the DNA containing the phaP promoter region (curve a), and barely bound to the DNA containing the phaR promoter region (curve b) and the control DNA (curve c). Figure 2B shows that the amount of the bound PhaR (Δm) followed a saturation curve as a function of the PhaR concentration ([PhaR]).
The E2 proteins of papillomavirus specifically bind to double-stranded DNA containing the consensus sequence ACCG-N4-CGGT, where N is any nucleotide.
In any event, the amount of DNA containing the StarLink gene that was found in most of the recalled foods was minimal.
But he wondered whether Bier's gene drive system could ferry the hefty 17,000 bases of DNA containing the mouse antibody genes.
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