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Here we describe the first crystal structure of a plant Fpg, Arabidopsis thaliana (AthFpg), unliganded and bound to DNA containing an abasic site analog, tetrahydrofuran (THF).
Chen made loops of bacterial DNA containing an integrase and a good copy of the PAH gene, and then injected these plasmids into a mouse's tail vein.
The same ATP effect was observed for pol V Mut WT synthesis on DNA containing an abasic site.
Results of the alignment revealed homology with the Vsr protein, which was involved in the mismatch repair of DNA containing an unpaired guanine in E. coli[ 28].
Both in vitro and in vivo studies have demonstrated preferences for nucleosomes to bind DNA containing an approximate 10-base periodicity of AA or TT dinucleotides [ 7- 9].
Serotonin immunoreactivity is restored in bas-1 mutants by DNA containing an intact C05D2.4 gene, but not with DNA mutated in C05D2.4.
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These two experimental studies provided evidence that DNA containing "A-T" base pairs can form both PS-DNA and APS-DNA.
The c-Abl DNA-binding domain interacts with deformed DNA structures such as four-way junctions and bubble DNA containing a large single-stranded loop,34 suggesting a role not only in transcription but also in recombination and DNA repair.
Pa-AGOG mutants K140Q, D172N and D172Q did bind GO containing single-stranded DNA more tightly than double-stranded DNA containing a GO/C base pair.
Using a double-stranded DNA containing a single U : G mismatch at low substrate concentration to mimic the in vivo situation, mSMUG1 was found to be the major UDG activity in extracts of cells from Ung−/− mice.
The system uses cell extracts of overexpressed P9 protein and empty procapsids from a P9-deficient mutant virus infection and PRD1 DNA containing a LacZα-insert.
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