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EMSA experiments demonstrated that CRTR-1-containing complexes could specifically bind DNA containing a CP2-response element (Figure 4A), as no competition for binding was observed using an oligonucleotide containing a mutated CP2-response element.
Recently, we developed an in vitro system using human uracil DNA glycosylase (UDG), AP endonuclease (APE), DNA polymerase β (pol β) and rotationally positioned DNA containing a single uracil associated with a 'designed' nucleosome, to test short-patch base excision repair (BER) in chromatin.
The c-Abl DNA-binding domain interacts with deformed DNA structures such as four-way junctions and bubble DNA containing a large single-stranded loop,34 suggesting a role not only in transcription but also in recombination and DNA repair.
Using a double-stranded DNA containing a single U : G mismatch at low substrate concentration to mimic the in vivo situation, mSMUG1 was found to be the major UDG activity in extracts of cells from Ung−/− mice.
Pa-AGOG mutants K140Q, D172N and D172Q did bind GO containing single-stranded DNA more tightly than double-stranded DNA containing a GO/C base pair.
The system uses cell extracts of overexpressed P9 protein and empty procapsids from a P9-deficient mutant virus infection and PRD1 DNA containing a LacZα-insert.
Double helical DNA, containing a π-stacked array of base pairs within its interior, can be considered as a molecular analogue of solid state π-stacked arrays.
To date, several structures of bacterial Fpg enzymes unliganded or in complex with DNA containing a damaged base have been published but there is no structure of a eukaryotic Fpg.
The present study provides proof of principle that canine muscle cells can be induced to produce and secrete canine insulin on transfection with nonviral plasmid DNA containing a novel mutant canine preproinsulin gene that produces furin-cleavable canine preproinsulin.
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These two experimental studies provided evidence that DNA containing "A-T" base pairs can form both PS-DNA and APS-DNA.
In 1988, the experimental strategies by Ramsing and Jovin confirmed that DNA containing A-T base pairs can exist as a stable parallel-stranded helix.
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