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Later, these DNA constructs were tested in mice to determine their adjuvant capability.
Site-directed mutagenesis was performed by PCR33 and the final DNA constructs were assembled in yeast by homologous recombination (see below).
DNA constructs were engineered such that the lacZ reporter gene was expressed under the control of portions of scleraxis regulatory regions.
These DNA constructs were co-expressed in HEK-293 cells and the assembly of heterogeneous triple-helical mini-procollagen VII molecules was analyzed.
All the DNA constructs were sequenced by GENEWIZ (Suzhou, China).
Primers for PCR and sequencing of DNA constructs were ordered from and performed by MWG (MWG-Biotech AG, Germany).
All of the DNA constructs were sequenced, and the molecular weights of the recombinant proteins were verified by mass spectrometry (MALDI).
DNA constructs were transfected 8 h.
All DNA constructs were verified by sequencing.
The nucleotide sequences of these DNA constructs were confirmed.
DNA constructs were created following the procedures in [34].
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