Exact(15)
Drew was a co-founder of Gen9, Inc., a DNA construction company; he returned to serve as a director while Gen9 was successfully acquired.
The system is compatible with Golden Gate cloning, which facilitates the DNA construction process and provides high design flexibility.
The first one is based on the codification of the possible solution in a binary string and in the application, in that string, of an assembly algorithm, such as one used in DNA construction.
And Bathe has added to that versatility: with graduate student Sakul Ratanalert, he recently developed software called DAEDALUS, which captures the complex rules of DNA construction in an algorithm that makes three-dimensional DNA design easier and more accessible to a wide range of scientists and engineers.
All the DNA construction steps were carried out in Escherichia coli TOP10 cells.
The first attempt to standardize DNA construction, NOMAD, was made 15 years ago [9].
Similar(45)
Being different from traditional DNA constructions, our method is based on algebraic number theory rather than classical heuristic algorithms and the conventional coding theory.
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
Many approaches have used viral particles carrying DNA constructions that can be integrated in the genome's cell randomly.
E. coli strain DH5α was used for plasmid DNA constructions and preparations using standard techniques as described [ 42] and were grown in LB broth supplemented with apramycin (50 μg/mL).
PIN3 is also responsible for the unique DNA conformer construction – a G-quadruplex structure, which overlaps intron 3 [ 12].
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